5 nm Gold-Ni-NTA Binds His Tags
نویسندگان
چکیده
منابع مشابه
Oligohis-tags: mechanisms of binding to Ni2+-NTA surfaces.
Since immobilized metal ion affinity chromatography (IMAC) was first reported, several modifications have been developed. Among them, Ni(2+) immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support has become the most common method for the purification of proteins carrying either a C- or N-terminal histidine (His) tag. Despite its broad application in protein purificat...
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Small molecule-based fluorescent probes have been used for real-time visualization of live cells and tracking of various cellular events with minimal perturbation on the cells being investigated. Given the wide utility of the (histidine)6-Ni(2+)-nitrilotriacetate (Ni-NTA) system in protein purification, there is significant interest in fluorescent Ni(2+)-NTA-based probes. Unfortunately, previou...
متن کامل2 Dta 2 Nta Nta
15 2DTA 2NTA NTA p MITL k U k DTA Figure 5: Relationship between various classes Theorem 5.2 The membership problem for 2NTA is solvable in PSPACE. Proof. Consider a 2NTA A, and let k be the magnitude of the largest constant appearing in a clock constraint in the transitions of A. Given a timed word w = (;) of length n, deene R w to be the set comprising of i + c and (i + j + c)=2 for 1 i; j n ...
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Highly monodisperse 5 nm Pd-Ni alloy nanoparticles (NPs) were prepared by the reduction of Pd(acac)(2)/Ni(acac)(2) mixtures with tert-butylamine-borane complex (TBAB) in the presence of oleic acid (OA) and oleylamine (OAm). Employing TBAB as an effective reductant and OA/OAm combination as an effective stabilizing agent is crucial to the formation of monodisperse Pd-Ni NPs. Experimental results...
متن کاملHis-tags lighten up and lose their inhibitions.
T he ability to control molecular behavior in the four dimensions of time and space is almost uniquely afforded by light. Consequently, there has been a long history of the use of light to trigger molecular interactions in the study of biological phenomena or the creation of active biomolecules (1). One application of this principle has been in the fabrication of protein microarrays, a subset o...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2005
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927605507712